API Staining Guide for Clear Nuclear Imaging DAPI staining is a widely used method for visualizing DNA within cells, offering sharp nuclear contrast in fluorescence microscopy. DAPI (4′,6-diamidino-2-phenylindole) is a fluorescent dye that binds strongly to A-T rich regions of double-stranded DNA, enabling clear visualization of cell nuclei. Beta LifeScience Whether you're preparing tissue sections, analyzing the cell cycle, or identifying nuclei in multi-color experiments, DAPI provides a reliable and easy approach. Below, we explore how it works, protocols, applications, and tips. What Is DAPI Staining? DAPI staining is a fluorescence labeling technique used to selectively stain DNA in cells. When DAPI binds to DNA, it emits bright blue fluorescence under UV light, making cell nuclei readily visible. Beta LifeScience Its strong affinity for A-T rich sequences and high fluorescence yield make DAPI a staple in histology, cell biology, and microscopy workflows. Excitatio...
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